Α-helical domains promote translocation of intrinsically disordered polypeptides into the endoplasmic reticulum*

HIGHLIGHTS

• who: Margit Miesbauer from the EXPERIMENTAL PROCEDURES Generation of PrP and PrP Mutants-The coding region of mouse prion protein gene (GenBankTM accession number, ) modified to express PrP-L M/V M was inserted into the mammalian expression vector pcDNA31/Zeo (Invitrogen), allowing detection by the monoclonal antibody (mAb) , . PrP was used as a template to introduce the following deletions and mutations by standard PCR cloning techniques. The generation of the constructs S X, ⌬27-, S X, ⌬27-156/S X, and mouse Doppel (Dpl) was described earlier (27-29). In PrP/ CHO, Q X/ CHO, W . . .
  

   

  

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