a dna barcode library for katydids, cave crickets, and leaf-rolling crickets (tettigoniidae, rhaphidophoridae and gryllacrididae) from zhejiang province, china

HIGHLIGHTS

• who: Yizheng Zhao from the DNA extraction and COI barcode region sequencing Total genomic DNA was extracted from hind legs of adults (N=676) and nymphs (N=5) using the Dneasy Blood and Tissue Kit (Tiangen Biotech, Beijing, China) according to the manufacturer`s specificationsThe remainder of the specimen was retained as a voucher stored at the Katydids Lab of Hebei University, China. The COI barcode region was amplified with primers COBU (5u00b4-TYT CAA CAA AYC AYA ARG ATA TTG, u00b4) and COBL (5u00b4-TAA ACT TCW GGR TGW CCA AAR AAT CA-3u00b4) (Pan et_al . . .
  

   

  

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