HIGHLIGHTS
SUMMARY
H. filiaventi" R50 is a chemolithotrophic microorganism that grows on H2, CO2 and O2 and produces large amounts of extracellular organic compounds, mainly amino_acids. Quantification of amino_acids An amino_acid standard mixture containing 17 different L-amino_acids at 1.25 and 2.5 μmoles/mL was obtained from Sigma Aldrich (AAS18-5 ml) and used to prepare calibration curves in blank culture medium for the Q-ToF LC-MS system described above. It was tested whether amino_acids could serve as energy and nitrogen source, but no growth was observed on the amino_acids such as alanine . . .
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