holothuria (lessonothuria) coronata sp. nov. (echinodermata, holothuroidea), a new species of sea cucumber from socorro island, méxico

HIGHLIGHTS

• who: Brenda Anaid Yu00e1u00f1ez Villanueva from the (anterior, medium, and posterior regions), dorsal papillae, ventral tube feet, and tentaclesThe tissue was dissolved in fresh household bleach (5-6.5%). After centrifugation at , rpm for , min, bleach was pipetted off and the ossicles were rinsed and centrifuged with distilled water that was pipetted off afterwards. The same process was done with, and, % ethanol. Absolute ethanol was added to the ossicles, and a small aliquot was taken and placed to dry on a cylindrical double-coated conductive carbon tape stub. Then, it was sputter coated with gold, ., kV in . . .
  

   

  

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