molecular and morphological evidence reveals a new genus of the subfamily heteropterinae (lepidoptera, hesperiidae) from china

HIGHLIGHTS

• who: Yongxiang Hou from the Laboratory protocols DNA was extracted from two or three legs of dried adult specimens using a TIANamp Genomic DNA Kit (Tiangen, Guangzhou, China) following the manufacturer's instructionsWe amplified a single mitochondrial gene (, bp of COI) and three nuclear genes (, bp of EF-1α, bp of RPS5, and , bp of Wingless), for a total of , bp. The primers used to amplify each gene were synthesised by Sangon Biotech (Shanghai, China) and are shown in Suppl. material, : Table, . DNA amplification was performed in, µL reaction volumes containing , µL of template DNA, ., µL of . . .
  

   

  

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