HIGHLIGHTS
SUMMARY
Chronic infection requires continuous mounting of an immune_response even in a tolerant species, thus incurring energy costs. All specimens were stored in 96% ethanol for detection of A. astaci DNA from crayfish cuticle as described in Suppl. material 1: S1, while in surviving crayfish from Experiment 2, hepatopancreas was additionally dissected for gene_expression analysis (described below). Relationships amongst gene_expression variables were further analysed using the principal component analysis. The first principal component Ana Dobrović et_al / NeoBiota 77: 155-177 is strongly correlated with three of the gene_expression variables CS, GAPDH and C/ EBP-β, suggesting . . .
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