thesium muasyae (santalaceae), a new species from the limestone fynbos of the overberg, south africa

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  • who: Daniel A. Zhigila from the Molecular work and barcoding Whole genome DNA was extracted from the silica-gel dried leaf materials collected during our fieldworks between , and The extraction was performed using a modified CTAB protocol (Doyle and Doyle, ) as amended by Zhigila et_al (2020). The following primers: ITS , (5u00b4-TCC TCC GCT TAT TGA TAT GC-3u00b4), (5u00b4GGA AGT AAA AGT CGT AAC AAG, u00b4) (White et_al, ), trnL-C (5u00b4-CGA AAT CGG TAG ACG CTA CG-3u00b4) and trnL-F (5u00b4-TT TGA ACT GGT GAC ACG AG-3u00b4) were used to amplify and . . .

     

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