HIGHLIGHTS
- who: Javier Isaac de la Fuente from the (UNIVERSITY) have published the article: Two new species of Trichoglossum (Geoglossaceae, Ascomycota) from south Mexico, in the Journal: (JOURNAL) of 01/Dec/2015
SUMMARY
DNA extraction, amplification, and sequencing Total DNA was extracted from silica-gel dried ascomata of the collected samples (specimens JF-208-ITCV and JF-526-ITCV), following a proteinase K protocol (Thermo Fisher Scientific, Waltham, MA, USA) according to Põldmaa et_al. Samples were incubated in 100 µl of lysis buffer and 2.5 μl of proteinase K at 56 °C . . .
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