3d molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

HIGHLIGHTS

  • who: Luca Pesce from the European LaboratoryUniversity of Florence, Sesto Fiorentino, Italy have published the research work: 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy, in the Journal: (JOURNAL) of 19/08/2021
  • what: Although SHIELD25 was fully applied to clear formalinfixed 2-mm-thick coronal blocks of the human brain (9 u00d7 5.5 u00d7 0.2 cm3) and label with different markers the replication of this approach showed a not homogeneous labelling for high densityepitopes. The tissue donors provided their informed and written consent to the donation of . . .

     

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