Prolyl-4-hydroxylase ώ subunit 2 (p4ha2) expression is a predictor of poor outcome in breast ductal carcinoma in situ (dcis)

HIGHLIGHTS

• who: Michael S. Toss from the , °C followed by the OptiView HQ Linker for , min and the OptiView HRP Multimer for , minCases with nuclear staining of more than, % of the tumour cells were considered positive. , status was assessed using IHC staining (1:400, DAKO, no antigen retrieval) with HercepTest scoring method as previously published [24, ]. Chromogenic in situ Hybridisation (CISH) was used to assess the , gene amplification to determine the final status of , within equivocal cases. , gene amplification was deemed positive where there were six or more signals per nucleus or when clusters were identified in the . . .
  

   

  

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