Repeatability and reproducibility of the forskolin-induced swelling (fis) assay on intestinal organoids from people with cystic fibrosis

HIGHLIGHTS

• What: The authors aimed to mitigate source for variation through sharing culture media and assay reagents where possible.
• Who: Marlou C. Bierlaagh from the Deptof Pediatric Pulmonology, Wilhelmina Children`s Hospital, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands have published the paper: Repeatability and reproducibility of the Forskolin-induced swelling (FIS) assay on intestinal organoids from people with Cystic Fibrosis, in the Journal: (JOURNAL)
• How: Briefly three weeks after thawing the PDIOs were tested for the following experi­ mental conditions (in duplicate) 1) non-treated (forskolin alone) or treated with 2 . . .
  

   

  

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