HIGHLIGHTS
- who: Masayuki Oginuma from the DepartmentOsaka University have published the paper: Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging, in the Journal: Scientific Reports Scientific Reports
- how: After incubation for one hour the authors selected embryos with strong red color in the whole cell body. (c) 9 days-post-fertilization (dpf) embryos uninjected or injected with single or triple sgRNAs.
SUMMARY
Arrow indicate PCR primers used for confirmation of knockout/deletion. (b) To make the whole-body biallelic knockout in N. furzeri, sgRNA#1 . . .
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