Discrepant phenotyping of monocytes based on cx3cr1 and ccr2 using fluorescent reporters and antibodies

HIGHLIGHTS

• What: The authors aimed to challenge this concept by antibody staining and reporter mouse models. As the first step, the authors aimed to set up a flow cytometry panel to characterize classical and non-classical monocytes based on Ly6C and CX3CR1, as suggested in the literature . In the next step, the authors aimed to verify whether the antibodies used were indeed functional and able to detect CX3CR1. The authors challenged the use of reporter mice and antibody staining in the current standard definition of mouse classical and non-classical monocytes as Ly6ChighCX3CR1lowCCR2high and Ly6ClowCX3CR1highCCR2-/low, respectively .
 



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