HIGHLIGHTS
- What: The primary reason is that conventional medium contained FBS, which is rich in cholesterol, and it was technically challenging to specifically remove cholesterol from it.
- Who: Pedro Lei and colleagues from the University at Buffalo, United States have published the Article: Development of a chemically disclosed serum-free medium for mouse pluripotent stem cells, in the Journal: (JOURNAL)
- How: These results indicated that cholesterol dose-dependently enhanced long-term mESC culture under serum-free media conditions and that the DA-X2iLIF/β-ME is therefore suitable for analyzing the metabolic requirements and mechanism . . .

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