Identification of tubular heparan sulfate as a docking platform for the alternative complement component properdin in proteinuric renal disease*

HIGHLIGHTS

• who: Azadeh Zaferani from the The Netherlands)The whole staining procedure was done at room temperature. Double staining of properdin and syndecan, was done on adriamycin-induced proteinuric rat renal tissue. The double staining was done by Zenon rabbit IgG labeling kit (Molecular Probes). Rabbit anti-human syndecan, was labeled with Alexa , with a molar ratio, :, of antibody/labeling reagent. Rabbit anti-human properdin was labeled with the same molar ratio with Alexa, . The antibodies were incubated with labeling reagents for , min and then blocked with the kit's blocking reagent for an additional , min, and the . . .
  

   

  

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