Potassium channel ä and β subunits assemble in the endoplasmic reticulum*

HIGHLIGHTS

• who: Naomi Nagaya from the (UNIVERSITY) have published the research: Potassium Channel ä and β Subunits Assemble in the Endoplasmic Reticulum*, in the Journal: (JOURNAL) of June/14,/1996

SUMMARY

Forty-eight h after transfection with Shaker alone, HEK 293T_cells were incubated for 30 min in methionine- and cysteine-free DMEM (Mediatech), pulsed for 3 h with 200 mCi/ml methionine and cysteine (Tran35S-Label, ICN or EasyTag Express Protein Labeling Mix, DuPont NEN), and chased for 2 h in complete, nonradioactive medium. In oocytes and HEK 293T_cells, glycosylation occurs in two stages, giving rise . . .

 



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