Efficient crispr/cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

HIGHLIGHTS

  • who: Remi L. Gratacap from the (UNIVERSITY) have published the article: Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system, in the Journal: (JOURNAL)
  • what: The authors developed an optimised protocol using lentivirus transduction for efficient integration of constructs into the genome of a Chinook salmon (Oncorhynchus tshwaytcha) cell line (CHSE-214). This approach has led to fundamental host-pathogen discoveries, particularly in virology as the cell intrinsic nature of the innate immune_response is very well suited to interrogation with this platform. The authors propose an optimised protocol . . .

     

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