Design and evaluation of a unique sybr green real-time rt-pcr assay for quantification of five major cytokines in cattle, sheep and goats

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  • who: Carinne Puech from the INRA, CMAEE, Montpellier, France have published the paper: Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats, in the Journal: (JOURNAL)
  • what: The aim of this study was thus to develop an assay to simultaneously quantify the of several cytokines and in three ruminant species. In the study , all primers were carried out fulfilling the criteria -with the exception of five primers that could not be designed to span exonexon boundaries-to achieve a GC . . .

     

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