Validation of a novel strategy for fluorescence quenching for a self-quenching fluorogenic probe and its application for visual loop-mediated isothermal amplification detection during food safety analysis

HIGHLIGHTS

• What: This study aims to identify an oligonucleotide sequence that is complementary to the and to employ the fluorescence mechanism of double-stranded DNA to establish a visualization system for the LAMP assay. 3 of 14 This study delves into the realm of self-quenching fluorogenic probes, with the exploration of various labeling types serving as the primary research focus. By constructing complementary sequences with distinct structural characteristics, the study compares the quenching efficiency of these sequences on the probe, aiming to illuminate the signal response mechanism underpinned by these complementary sequences. The high background signal is . . .
  

   

  

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