H7n7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitro

HIGHLIGHTS

• What: The aim of the study was to elucidate sex-specific responses of microglia and neurons to IAV infection. For the analysis of microglia morphology and activation status, z-stacks with an increment interval of 0.25 µm were acquired using the 20X objective (NA 0.8). For the analysis of dendritic spines, images of primary neurons expressing citrine and stained against PSD95 and synaptophysin were acquired using a Leica TCS SP8 STED microscope (HC PL APO CS2 93x oil objective, NA 1.30, pixel size 102 nm) and the Leica Application Suite X (LASX). The study . . .
  

   

  

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