HIGHLIGHTS
- who: Siau Jia Wei and collaborators from the , Germany, School of, Nanyang Technological University, Singapore, Singapore, Department of have published the Article: In Vitro Selection of Mutant HDM2 Resistant to Nutlin Inhibition, in the Journal: PLOS ONE | www.plosone.org of April/30,/2013
SUMMARY
The library was ligated into 2ConA-PET22b via Nde1/BamH1 sites and re-amplified with petF2 and petR to make library amplicons with T7 promoter and ribosome binding_site required for in_vitro transcription-translation (IVT), as well as the 2ConA RE site located before the T7 promoter site. Magnetic . . .
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