A systematic analysis of affinity tags in the haloarchaeal expression system, haloferax volcanii for protein purification

HIGHLIGHTS

• What: In the study, H. volcanii strain H1895 as the host, in combination with the pTA1992 plasmid that features the exceptionally strong constitutive p.syn promoter (Haque et_al, 2019, 2020) was employed. L: Low salt (200 mM NaCl); H: High salt (2 M NaCl) in the binding, washing, and elution buffers. the study reveals that N-terminal Strep-tag II results in approximately five times higher expression, suggesting that C-terminal usage in H. volcanii should be reconsidered (Braun Frontiers in Microbiology et_al, 2019). This approach has proven effective not just for sfGFP but also for mCherry . . .
  

   

  

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