Α-galactosidase and sucrose-kinase relationships in a bi-functional agask enzyme produced by the human gut symbiont ruminococcus gnavus e1

HIGHLIGHTS

  • who: Thierry Giardina from the Wageningen University and Research, Argentina have published the Article: α-Galactosidase and Sucrose-Kinase Relationships in a Bi-functional AgaSK Enzyme Produced by the Human Gut Symbiont Ruminococcus gnavus E1, in the Journal: (JOURNAL)
  • how: Regarding ATP hydrolysis the results showed that in both cases the enzymes presented Michaelian kinetics .

SUMMARY

    Expression and Purification of the Recombinant RgAgaSK and Its Truncated Variants To investigate the interactions between both domains of the RgAgaSK enzyme, the authors generated two variants consisting of each domain individually expressed in Escherichia coli . . .

     

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