Infection of novel reassortant h1n2 and h3n2 swine influenza a viruses in the guinea pig model

HIGHLIGHTS

• who: Rodrigo Tapia from the Center for Research on Influenza Pathogenesis (CRIP), Icahn School of Medicine at Mount Sinai, New York City, NY, USABriefly, all viral segments were amplified by a multisegment reverse transcription-PCR (M-RTPCR), following a previously described protocol [17]. Multiplexed libraries were prepared and sequenced on an Illumina HiSeq , platform in a single-end , nt run format, and the viral genomes were assembled de novo. The neuraminidase (NA) segment was evaluated by the same phylogenetic analysis previously described for the HA segment. Internal gene sequences were submitted to BLAST to reveal the closest . . .
  

   

  

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