Neurotoxic, redox-competent alzheimer’s β-amyloid is released from lipid membrane by methionine oxidation*

HIGHLIGHTS

• who: Kevin J. Barnham from the at , °CThe solution was decanted from the glass beads and subjected to five freeze-thaw cycles in liquid nitrogen and a , °C water bath. The lipids were then extruded , times through a, .2-␮m pore filter from Millipore, using an Avanti mini-extruder apparatus. LUV were stored at , °C and used within , h of preparation as described by Hope et_al (43). Peptides as a freeze-dried powder were added to the desired concentration to a suspension of LUV in buffer, and the mixture was vortexed under , for , min at , K in . . .
  

   

  

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