Targeted inhibition of ca2+/calmodulin-dependent protein kinase ii in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17*

HIGHLIGHTS

• who: Yong Ji from the Generation of CaMKII-, Transgenic Mice-A synthetic gene expression unit was engineered to encode three functional domains using oligonucleotides produced by the DNA Core Facility at the University of CincinnatiStarting at the amino terminus, the expression unit contains nucleotides that will encode a CaMKII inhibitor, a FLAG epitope, and an SR localization signal. The CaMKII inhibitor domain consisted of sequences encoding a tetramer of the CaMKII autocamitide inhibitory peptide (AIP4), this, amino acid sequence (KKALRRQEAVDAL) is known to be a highly specific and potent inhibitor of CaMKII (25). Sequences encoding a FLAG . . .
  

   

  

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