HIGHLIGHTS
- who: Asad U. Khan from the Lille University of Science and Technology, France have published the research work: CRISPRi-mediated suppression of E. coli Nissle 1917 virulence factors: A strategy for creating an engineered probiotic using csgD gene suppression, in the Journal: (JOURNAL)
- what: The central hypothesis of the study was to design the probiotic E. coli Nissle 1917 (EcN) strain with minimized curli amyloid and other virulence factors productions by editing the csgD gene using CRISPR interference (CRISPRi).
SUMMARY
CRISPRi geneediting system was used to inhibit the csgD gene_expression. Several . . .
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