Interaction of the mammalian endosomal sorting complex required for transport (escrt) iii protein hsnf7-1 with itself, membranes, and the aaa+ atpase skd1*

HIGHLIGHTS

• who: Yuan Lin from the Plasmids and Mutagenesis-To express , in Escherichia coli, mouse , cDNA (a kind gift from DrCarol Vandenberg (University of California)) was inserted into pHO4d (41) between NcoI and EcoRI sites with amino acids PNSG between the C terminus of , and the His6-Myc tag. The E Q mutation was generated by QuikChangeTM site-directed mutagenesis (Stratagene). To express , wild type or E Q in mammalian cells, the corresponding cDNA was inserted into pEGFP-, (Clontech) between XhoI and BamHI sites. The resulting proteins have amino acids ADPPVAT between , and EGFP. To create tetracycline-regulated . . .
  

   

  

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