Molecular structure of ù±-d-glucose-1-phosphate cytidylyltransferase from salmonella typhi*

HIGHLIGHTS

• who: Nicole M. Koropatkin from the Cloning of the Glucose-1-phosphate Cytidylyltransferase Gene-The sequence for the gene encoding glucose-1-phosphate cytidylyltransferase, rfbF, has been reported and allowed for the design of primers for gene amplification (21)Genomic DNA for S. typhi isolates CDC numbers, and , was obtained as a generous gift from the laboratory of Dr. Stanley Maloy at the University of Illinois at UrbanaChampaign. The rfbF gene was PCR amplified from genomic DNA such that the forward primer, ⬘-CTAGCTAGCAAAGCGGTCATCCTGGCTGGT-3⬘ included an NheI site, whereas the reverse primer, ⬘-CCCAAGCTTTTACTCCCAGGTTTTCCACGGAGC-3⬘ included a HindIII . . .
  

   

  

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