Molecular dissection of phage endolysin

HIGHLIGHTS

• who: Amol Arunrao Pohane from the Table , lists the oligos along with their sequencesThe mycobacteriophage , was a kind gift from Graham Hatfull (University of Pittsburgh) and was cultured following the published protocol (22). The genomic DNA of the phage was isolated as described (27). Plasmid purification, restriction digestion, ligation, and transformation were carried out as described (27). Site-directed mutagenesis was carried out following the single primer method (28, ). The gp, gene was PCR-amplified using a set of primers and , genomic DNA as template. For its expression in E. coli, cloning was carried out in pET21b . . .
  

   

  

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