Gcn2 mediates gcn4 activation in response to glucose stimulation or uv radiation not via gcn4 translation*

HIGHLIGHTS

• who: Irit Marbach from the instead of, % (42)Cultures were further grown for about , h. Then glucose (or other sugars as indicated in particular experiments) were added from a stock solution of, % to reach concentration of, %. Samples were collected by centrifugation at the indicated time points, frozen immediately in liquid nitrogen, and stored at ⫺, °C prior to RNA or lysate preparations or frozen at ⫺, °C prior to ␤-galactosidase assay. Yeast cultures were exposed to UV radiation as previously described (24). RNA Preparation and Analysis-Frozen pellets were thawed on ice, and total RNA was prepared and analyzed . . .
  

   

  

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