HIGHLIGHTS
- who: D. Balemans from the These experiments were performed at Queen Mary University of LondonExperiments were performed using colons from , weeks old male C BL/, mice (Charles River UK, Ltd.). Single unit afferent nerve activity was recorded from teased lumbar splanchnic nerve fibers innervating the distal colon, as previously described33. Receptive fields were identified and characterized based on the criteria previously developed by Brierley et_al34, . Only colonic afferents with presumptive nociceptor function were used. Mechanosensitivity was determined by repeated probing of the receptive field with a, ., g von Frey hair (vFh). Thereafter, rectal biopsy supernatants (, µl) were . . .
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