Acute and long-term effects of antibiotics commonly used in laboratory animal medicine on the fecal microbiota

HIGHLIGHTS

• who: Scott W. Korte from the Extracted fecal DNA was processed at the University of Missouri DNA Core FacilityBacterial S rDNA amplicons were constructed via amplification of the , region of the , s rDNA gene with universal primers (U F/ R) previously developed against the , region, flanked by Illumina standard adapter sequences [11, ]. Oligonucleotide sequences are available at proBase [13]. A single forward primer and indexed reverse primers were used in all reactions. PCR was performed in , µL reactions containing , ng metagenomic DNA, primers (0., µM each), dNTPs (, µM each), and Phusion high-fidelity DNA polymerase ( U). Amplification . . .
  

   

  

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