HIGHLIGHTS
- who: Sai Li from the Cdt Cdt, was cloned into a pET16b vector followed by replacement of the NcoI/NdeI region with insertion of a DNA segment encoding a, × Flag tag. E. coli was transformed with the pFlag-Cdt1-pET plasmid and cells were grown in LB + , µg/ml ampicillin at , °C with shaking until reaching an , of, .6, at which time the culture temperature was quickly reduced to , °C by shaking in an ice bath. Then IPTG (, mM) was added to induce Cdt, expression for , h at , °C. Cells were then harvested by low speed centrifugation . . .
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