Forkhead box m1 is essential for nuclear localization of glioma-associated oncogene homolog 1 in glioblastoma multiforme cells by promoting importin-7 expression*

HIGHLIGHTS

• who: Jianfei Xue from the Cell Culture and Transfection-, knock-out mouse embryonic fibroblasts and HEK T human kidney, Hs, glioma, anaplastic astrocytoma, glioblastoma, and , glioblastoma cells were grown in Dulbecco's modified Eagle's medium containing, % fetal bovine serum (HyClone, Logan, UT)HEK T cells were transfected with FuGENE HD (Roche Applied Science). , knock-out mouse embryonic fibroblasts and Hs683, HFU251, and , cells were transfected with, tremeGENE HP (Roche Applied Science). , and , cells were transfected with siRNA using Lipofectamine RNAiMAX (Invitrogen). Plasmids, siRNAs, and Reagents-Human , and , mutants were generated via PCR and using a QuikChange . . .
  

   

  

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