Improved methods of carnivore faecal sample preservation, dna extraction and quantification for accurate genotyping of wild tigers

HIGHLIGHTS

• who: Patlolla Anuradha Reddy. and colleagues from the Centre for Cellular and Molecular Biology, Hyderabad, India have published the paper: Improved Methods of Carnivore Faecal Sample Preservation, DNA Extraction and Quantification for Accurate Genotyping of Wild Tigers, in the Journal: PLOS ONE | www.plosone.org of June/18,/2012
• what: The authors show a strong relation of PCR success rate to initial amounts of DNA used.
• how: After ascertaining optimal amounts of template DNA to avoid genotyping errors in felid faecal extracts the authors compared and adopted several pre-PCR protocols to advance . . .
  

   

  

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