Crispr/cas9-mediated sarm1 knockout and epitope-tagged mice reveal that sarm1 does not regulate nuclear transcription, but is expressed in macrophages

HIGHLIGHTS

• who: Ciara G. Doran from the C with, % CO To generate primary BMDM (pBMDM), bone marrow from the tibiae and femurs from mice was flushed using a syringe, red blood cells were lysed, and the resulting cells were cultured in complete DMEM supplemented with, % (v/v) , supernatant as a source of, CSF (23). On day, cells were removed from dishes using a sterile cell scraper and seeded for experiments in complete DMEM supplemented with, % (v/v) , supernatant. Immortalized BMDMs (iBMDM) were generated with , recombinant retrovirus carrying ν, myc and ν, raf/mil oncogenes (48). , recombinant retrovirus was kindly provided . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.