Identification of a fungal 1,8-cineole synthase from hypoxylon sp. with specificity determinants in common with the plant synthases*

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  • who: Jeffrey J. Shaw from the EXPERIMENTAL PROCEDURES Fungal Isolation and Culturing-Plant stem material was collected from various locations in Ecuador as well as Kubah National Park in Sarawak, MalaysiaStems were stored in clean plastic bags and transported back to Yale University, where they were surface-sterilized and plated on isolation medium comprised of, ⫻ potato dextrose agar (PDA) (, g/liter potato dextrose), .1⫻ PDA (2., g/liter potato dextrose) (EMD Millipore), or water agar (all , g/liter agar). Several fungi were isolated in the presence of, day-old Muscodor albus, which was used to enrich for . . .

     

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