In vitro polymerization of heparan sulfate backbone by the ext proteins*

HIGHLIGHTS

• who: Marta Busse from the stable EXT1p , clones was transfected with pSeqTagC EXT2, and double EXT1/, transfectants were selected by addition of , ␮g/ml Zeocin (Invitrogen)The stable clones were maintained in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with, % (v/v) fetal calf serum (Invitrogen), ␮g streptomycin sulfate/ml, units penicillin G/ml, and , ␮g geneticin/ml or , ␮g geneticin/ml together with , ␮g/ml Zeocin. To isolate expressed proteins, the medium was changed to serum-free Optimem, with Glutamax-I (Invitrogen). Conditioned medium was collected after , h, and protein expression was monitored by Western . . .
  

   

  

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