Targeting of protein kinase a by muscle a kinase-anchoring protein (makap) regulates phosphorylation and function of the skeletal muscle ryanodine receptor*

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• who: Mary L. Ruehr from the Construction of Vectors for the Expression of ECFP-tagged mAKAP or mAKAP-P-Cloning of mAKAP is described by Kapiloff et_al (14)Full-length mAKAP or a mutant form of mAKAP, mAKAP-P (I P), which does not bind PKA, was ligated into pEGFP to obtain green fluorescent fusion proteins (14). The substitution of a proline for an isoleucine disrupts the amphipathic helix of the RII-binding domain of mAKAP (I P), thus abolishing RII binding. The cDNA coding for EGFP was excised from mAKAP-EGFP or mAKAP-P-EGFP using . . .
  

   

  

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