Orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor ii (coup-tfii) protein negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation through suppressing runt-related gene 2 (runx2) activity*

HIGHLIGHTS

• who: Kkot-Nim Lee from the EXPERIMENTAL PROCEDURES Materials-Recombinant human , protein was purchased from RandD Systems (Minneapolis, MN)Antibody against COUP-TFII was from Abcam (Cambridge, UK), and antibody against Runx, was from Santa Cruz Biotechnology (Santa Cruz, CA). Hemagglutinin (HA) antibody was from Sigma-Aldrich. Actin and Myc antibodies were obtained from Cell Signaling Technology (Beverly, MA). Adenoviral (Ad) vector expressing COUP-TFII and HA-tagged COUP-TFII expression vector were kindly provided by Dr. Kee-Sook Lee (Chonnam National University, Gwangju, Korea). Ad-, and AdRunx, were kindly provided by Dr. Renny Franceschi (University of . . .
  

   

  

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