HIGHLIGHTS
- who: Oksana A. Sergeeva from the (UNIVERSITY) have published the research work: Biochemical Characterization of Mutants in Chaperonin Proteins CCT4 and CCT5 Associated with Hereditary Sensory Neuropathy*, in the Journal: (JOURNAL) of July/27,/2014
- what: The human ␥D-crystallin aggregation suppression and refolding assay used in this study has been used for many other chaperonins .
- how: For this assay the authors used an mHtt protein that was GST-tagged and contained a TEV protease site.
SUMMARY
Sucrose gradient centrifugation revealed chaperonin-sized complexes of both WT and mutant . . .
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