Anti-apoptotic molecule bcl-2 regulates the differentiation, activation, and survival of both osteoblasts and osteoclasts*

HIGHLIGHTS

• who: Yuichi Nagase from the subjected to SDS-PAGE with, % Tris-glycine gradient gel or, % Tris-glycine gel and transferred onto nitrocellulose membranes (Bio-Rad). After blocking with, % milk/TBS-T, membranes were incubated with primary antibodies to Bcl, (Pharmingen), activated caspase, (Cell Signaling Technology), or ␤-actin (Sigma), followed by horseradish peroxidase-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (Promega). Immunoreactive bands were visualized with ECL Plus (Amersham Biosciences), according to the manufacturer's instructions. The blots were stripped by incubating for , min in stripping buffer (2% SDS, mM, mercaptoethanol, and, ., mM Tris-HCl . . .
  

   

  

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