Phosphorylation of slit diaphragm proteins nephrin and neph1 upon binding of hgf promotes podocyte repair

HIGHLIGHTS

• who: Ashish K. Solanki from the confirm transfectionThe plasmids were commercially obtained from Santa Cruz Biotechnology (Cat No. sc- , and sc-400101-HDR-2). For complete allelic knockouts stable clones grown from single cells were isolated. The KO in the fully selected clones was confirmed by Western blotting using the MET-specific antibody. Drosophila nephrocytes as a model for HGF-induced renoprotection The Drosophila experiments were designed and performed at the University of Maryland by Dr Wen and Dr Han. Briefly, Drosophila pericardial nephrocytes were dissected in artificial hemolymph and then treated under different conditions for , min . . .
  

   

  

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