An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography

HIGHLIGHTS

  • who: Agata Butryn from the Didcot, UKUniversity of have published the research: An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
  • what: The authors show an alternative on-demand droplet-based mixing strategy for time-resolved mixing experiments.
  • how: Each dataset was collected within 30-80 min consuming between 162 and 420 µL of microcrystal slurry per one dataset which corresponds to estimated average sample consumption of 1.40 and 0.27 µg of HEWL and CTX-M-15 per integrable . . .

     

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