A parasite cysteine protease is key to host protein degradation and iron acquisition∗

HIGHLIGHTS

• who: Theresa C. O'Brien from the h at , °CAll subsequent washes were carried out with excess, PBS. Fixed cells were washed and applied to, mm round coverslips that had been coated with polylysine (0.1% w/v in water, Sigma) and allowed to settle for , min at room temperature. The cells were permeabilized in, PBS containing, .1% Triton , (Sigma) for , min, washed, and blocked for , h with, % bovine serum albumin (BSA) prepared in, PBS. After blocking, cells were incubated in rabbit anti-p, antiserum (a gift from J. D. Bangs) (9) (diluted, :, in, % BSA/D-PBS . . .
  

   

  

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