Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway*

HIGHLIGHTS

• who: Collynn F. Woeller from the TCATTAGGAGGGAGAGTAACCGGCA-3⬘), and L F (5⬘-GAGAGCTTCGCCTCTTTCTTCCCTCTGCCTGGCCTG-3⬘The mutations are shown in boldface type, and the modified codons are underlined. The corresponding complement primers were also used following the protocol for the site-directed mutagenesis kit (Stratagene). All cDNAs were sequence-verified at the Cornell BioResource Center. Quantitative ␤-Galactosidase Assay-Plasmids containing either the cSHMT, cSHMT-K R, cSHMT-K R/K R, or cSHMT-L F cDNA fused to the DNA binding domain of Gal, were cotransformed into , yeast with a plasmid encoding , fused to the Gal, activating domain . . .
  

   

  

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