Pyruvate dehydrogenase kinase-4 structures reveal a metastable open conformation fostering robust core-free basal activity*

HIGHLIGHTS

• who: R. Max Wynn from the EXPERIMENTAL PROCEDURES Reagents and Recombinant Proteins-Compound , (45) was a generous gift from DrRachel Mayers, Astrazeneca, UK. Dichloroacetate was purchased from Sigma. Human PDK isoforms (1-3) were expressed either as, terminal His, tagged small ubiquitin modifier (SUMO) or MBP (maltose-binding protein) fusions with a tobacco etch virus (TEV) protease recognition sequence (Glu-Asn-Leu-Tyr-Phe-Gln Gly, the arrow showing the cleavage site) engineered into the linker region as described previously (23, ). His, tagged human , (residues, ), (residues , ), and , (residues, ) were expressed also as described previously (23). The expression . . .
  

   

  

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