Yeast two-hybrid system demonstrates that estrogen receptor dimerization is ligand-dependent in vivo(∗)

HIGHLIGHTS

• who: Hong Wang from the cDNA and Constructs Construction of GAL4-hER fusion vectors involved cloning human ER cDNA into the p, (, DB) and p, (, TA) fusion vectors (kindly provided by DrsChevray and Nathans). Human ER cDNA (hER) was kindly provided by Dr. Katzenellenbogen, which was placed into the SalI site of p, (Dr. David Russell, University of Texas Southern Medical Center) (29). We subcloned the hER cDNA digested with SalI into pBluescript II , at the SalI site such that its transcription is dependent on , polymerase (T7-hER)., hER was amplified with , primer and an oligonucleotide (59 . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.