High affinity s-adenosylmethionine plasma membrane transporter of leishmania is a member of the folate biopterin transporter (fbt) family*

HIGHLIGHTS

• who: Larbi Dridi from the parameters were determined by linear regression analysis and Michaelis-Menten analysisThe transport competition study was performed with , ⫻ , LV SNF4000., cells expressing the AdoMetT1-GFP protein. Briefly, cells were incubated for , min in an assay buffer, as described previously (31), containing , nM radioactive AdoMet in the presence or absence of various concentrations (, nM and , and , ␮M) of competing molecules. For all transport experiments, the accumulation in cells incubated on ice was subtracted. Quantitative Real Time Reverse Transcription-PCR-Three independent RNA preparations were used for each real time PCR experiment. RNA extractions, cDNA . . .
  

   

  

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